Scoop has an Ethical Paywall
License needed for work use Register

Video | Business Headlines | Internet | Science | Scientific Ethics | Technology | Search


Nano-sized Probes Reveal How Cellular Structure Responds To Pressure

A new technique allows researchers to map how the cellular 'skeleton' adapts to external stress.

TSUKUBA, Japan, Nov 21, 2023 - (ACN Newswire) - By giving living cells a ‘nano-poke’ and monitoring the resulting changes in the intra-cellular environment, researchers have gotten their first glimpse of how whole cells respond to external mechanical pressure.

How foot stress (prestress) distribution varies with foot function.

A team lead by scientists from the National Institute for Materials Science in Tsukuba, Japan, used a technique called atomic force microscopy to apply force across the surface of various cells. The method uses nanoscale probes, with tips just a few billionths of a metre in size, to measure and map how force gets distributed across the cellular surface and throughout the cell. The researchers used machine learning to analyse and model the forces they measured. They also used fixing and staining techniques to study how the force distortion affected the cell’s internal structures and the microtubules and actin filaments that make up its ‘skeleton’.

“Cells are smart materials that can adapt to various chemical and mechanical stimuli from their surroundings,” says Jun Nakanishi, one of the corresponding authors of the study and the leader of the Mechanobiology Group at the National Institute for Materials Science. That ability to adapt relies on rapid feedback mechanisms to keep the cell intact and healthy, and there’s growing evidence that the failure of this cellular response underlies a range of ailments, including diabetes, Parkinson’s disease, heart attacks, and cancer.

Advertisement - scroll to continue reading

Are you getting our free newsletter?

Subscribe to Scoop’s 'The Catch Up' our free weekly newsletter sent to your inbox every Monday with stories from across our network.

So far, studies of these cellular responses have been limited by the techniques used – for example, some methods require that cells be pre-fitted with sensors, so they can only measure a small part of the response. “We invented a unique way to ‘touch’ a cell with nanoscale ‘hand’, so that the force distribution over a complete cell could be mapped with nanometer resolution,” says Hongxin Wang, who is the first author of the study and JSPS postdoc in the Mechanobiology Group.

The study revealed that tensional and compressional forces are distributed across actin fibres and microtubules within the cell to keep its shape, similar to how the poles and ropes of a camping tent work. When the researchers disabled the force-bearing function of actin fibres, they found that the nucleus itself is also involved in counterbalancing external forces, highlighting the role of the internal structure of the nucleus in the cellular stress response.

The research team also compared the responses of healthy and cancerous cells. Cancer cells proved more resilient to external compression than the healthy cells, and they were less likely to activate cell death in response.

The findings not only illuminate the complex intracellular mechanics of the stress response, but the discovery of different responses in cancer cells could offer a new way to distinguish healthy and cancerous cells – a diagnostic tool based on cellular mechanics.

Hospitals currently use the size, shape, and structure of a cell in diagnosing cancer. However, these features don’t always provide enough information to tell the difference between healthy and diseased cells. “Our findings provide another way of checking cell conditions by measuring force distribution, which could dramatically improve diagnostic accuracy,” says Han Zhang, another corresponding author of the study and the senior researcher of the Electron Microscopy Group, NIMS.

The study was published in the journal Science and Technology of Advanced Materials.

© Scoop Media

Advertisement - scroll to continue reading
Business Headlines | Sci-Tech Headlines

Digitl: Regulatory Challenges For Telecomms

The Download Weekly sits down with the Telecommunications Commissioner and members of the Commerce Commission team overseeing industry regulation to get a better idea of the watchdog’s thinking on key issues. “The most telling thing here is that the market share of the smaller providers has increased marginally year-on-year in the past twelve months," says Gilbertson. More

IAG: Call On New Government To Prioritise Flood Resilience

The economic toll of our summer of storms continues to mount, with insurance payouts now topping $1B (second only to the Christchurch earthquakes). AMI, State, & NZI have released the latest Wild Weather Tracker, which reveals 51,000 claims for the North Island floods & Cyclone Gabrielle, of which 99% (motor), 97% (contents), and 93% (home) of claims have now been settled. More


Join Our Free Newsletter

Subscribe to Scoop’s 'The Catch Up' our free weekly newsletter sent to your inbox every Monday with stories from across our network.